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hanks balanced salt solution hbss  (Thermo Fisher)


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    Structured Review

    Thermo Fisher hanks balanced salt solution hbss
    Hanks Balanced Salt Solution Hbss, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hbss+without+calcium/Hanks'+Balanced+Salt+Solution+(1X)%2C+without+calcium%2C+without+magnesium%2C+without+phenol+red/pmc13052091-33-0-13
    Average 97 stars, based on 1 article reviews
    hanks balanced salt solution hbss - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Virus:

    Article Title: Fasciola hepatica products can alter the response of bovine immune cells to Mycobacterium avium subsp. paratuberculosis
    Article Snippet: Following euthanasia by intra‐jugular administration of pentobarbital sodium (Euthanal 200 mg/mL, Merial Animal Health), lymph nodes were extracted during post‐mortem examination and suspended in ice‐cold Hank’s balanced salt solution (HBSS; without calcium or magnesium) supplemented with 1% antibiotic–antimycotic solution (Thermo Fisher Scientific).

    Article Title: STING signaling sensing of DRP1-dependent mtDNA release in kupffer cells contributes to lipopolysaccharide-induced liver injury in mice
    Article Snippet: A 26G catheter was inserted into the mouse portal vein, and the liver was perfused with 30 mL warmed Hank’s balanced salt solution (HBSS, without calcium or magnesium, GIBCO) containing 1% penicillin streptomycin to flush out the blood, followed by 40 mL DMEM/F12 (GBICO) containing 0.05% collagenase IV (GBICO) and 1% penicillin streptomycin.

    Article Title: NeuriteNet: A Convolutional Neural Network for determining morphological differences in neurite growth
    Article Snippet: Lumbar DRGs were harvested into NeurobasalTM media (Cat #21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), Cat #17100, ThermoFisher) for 40 min. After addition of 10% FBS to inactivate the proteases, the cell suspension was centrifuged at 201 rcf for 5 min.

    Article Title: Caldendrin represses neurite regeneration and growth in dorsal root ganglion neurons
    Article Snippet: DRGs (20–30) were harvested in NeurobasalTM media (Cat#21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin–EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), (Cat #17100, ThermoFisher) for 40 min. After the enzyme incubation, 10% horse serum was added to inactivate the proteases, the cell suspension was centrifuged at 500 rcf for 5 min.

    Article Title: NeuriteNet: A convolutional neural network for assessing morphological parameters of neurite growth
    Article Snippet: Lumbar DRGs were harvested into NeurobasalTM media (Cat #21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), Cat #17100, ThermoFisher) for 40 min. After addition of 10% FBS to inactivate the proteases, the cell suspension was centrifuged at 168 × g for 5 min.

    Article Title: Caldendrin represses neurite regeneration via a sex-dependent mechanism in sensory neurons
    Article Snippet: 20-30 DRGs were harvested into NeurobasalTM media (Cat#21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (-/-)), (Cat #17100, ThermoFisher) for 40 min. After the enzyme incubation, 10% horse serum was added to inactivate the proteases, the cell suspension was centrifuged at 500 rcf for 5 min.

    Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
    Article Snippet: Either HBSS with Calcium (14025092, Thermo Fisher Scientific Inc., Waltham, MA, USA) or without Calcium (14175095, Thermo Fisher Scientific Inc., Waltham, MA, USA) were used in the respective experiments, further supplemented with 2mM CaCl 2 or with 2 mM EGTA.

    Generated:

    Article Title: Fasciola hepatica products can alter the response of bovine immune cells to Mycobacterium avium subsp. paratuberculosis
    Article Snippet: Following euthanasia by intra‐jugular administration of pentobarbital sodium (Euthanal 200 mg/mL, Merial Animal Health), lymph nodes were extracted during post‐mortem examination and suspended in ice‐cold Hank’s balanced salt solution (HBSS; without calcium or magnesium) supplemented with 1% antibiotic–antimycotic solution (Thermo Fisher Scientific).

    Article Title: STING signaling sensing of DRP1-dependent mtDNA release in kupffer cells contributes to lipopolysaccharide-induced liver injury in mice
    Article Snippet: A 26G catheter was inserted into the mouse portal vein, and the liver was perfused with 30 mL warmed Hank’s balanced salt solution (HBSS, without calcium or magnesium, GIBCO) containing 1% penicillin streptomycin to flush out the blood, followed by 40 mL DMEM/F12 (GBICO) containing 0.05% collagenase IV (GBICO) and 1% penicillin streptomycin.

    Article Title: NeuriteNet: A Convolutional Neural Network for determining morphological differences in neurite growth
    Article Snippet: Lumbar DRGs were harvested into NeurobasalTM media (Cat #21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), Cat #17100, ThermoFisher) for 40 min. After addition of 10% FBS to inactivate the proteases, the cell suspension was centrifuged at 201 rcf for 5 min.

    Article Title: Caldendrin represses neurite regeneration and growth in dorsal root ganglion neurons
    Article Snippet: DRGs (20–30) were harvested in NeurobasalTM media (Cat#21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin–EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), (Cat #17100, ThermoFisher) for 40 min. After the enzyme incubation, 10% horse serum was added to inactivate the proteases, the cell suspension was centrifuged at 500 rcf for 5 min.

    Article Title: NeuriteNet: A convolutional neural network for assessing morphological parameters of neurite growth
    Article Snippet: Lumbar DRGs were harvested into NeurobasalTM media (Cat #21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), Cat #17100, ThermoFisher) for 40 min. After addition of 10% FBS to inactivate the proteases, the cell suspension was centrifuged at 168 × g for 5 min.

    Article Title: Caldendrin represses neurite regeneration via a sex-dependent mechanism in sensory neurons
    Article Snippet: 20-30 DRGs were harvested into NeurobasalTM media (Cat#21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (-/-)), (Cat #17100, ThermoFisher) for 40 min. After the enzyme incubation, 10% horse serum was added to inactivate the proteases, the cell suspension was centrifuged at 500 rcf for 5 min.

    Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
    Article Snippet: Either HBSS with Calcium (14025092, Thermo Fisher Scientific Inc., Waltham, MA, USA) or without Calcium (14175095, Thermo Fisher Scientific Inc., Waltham, MA, USA) were used in the respective experiments, further supplemented with 2mM CaCl 2 or with 2 mM EGTA.

    Recombinant:

    Article Title: Fasciola hepatica products can alter the response of bovine immune cells to Mycobacterium avium subsp. paratuberculosis
    Article Snippet: Following euthanasia by intra‐jugular administration of pentobarbital sodium (Euthanal 200 mg/mL, Merial Animal Health), lymph nodes were extracted during post‐mortem examination and suspended in ice‐cold Hank’s balanced salt solution (HBSS; without calcium or magnesium) supplemented with 1% antibiotic–antimycotic solution (Thermo Fisher Scientific).

    Article Title: STING signaling sensing of DRP1-dependent mtDNA release in kupffer cells contributes to lipopolysaccharide-induced liver injury in mice
    Article Snippet: A 26G catheter was inserted into the mouse portal vein, and the liver was perfused with 30 mL warmed Hank’s balanced salt solution (HBSS, without calcium or magnesium, GIBCO) containing 1% penicillin streptomycin to flush out the blood, followed by 40 mL DMEM/F12 (GBICO) containing 0.05% collagenase IV (GBICO) and 1% penicillin streptomycin.

    Article Title: NeuriteNet: A Convolutional Neural Network for determining morphological differences in neurite growth
    Article Snippet: Lumbar DRGs were harvested into NeurobasalTM media (Cat #21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), Cat #17100, ThermoFisher) for 40 min. After addition of 10% FBS to inactivate the proteases, the cell suspension was centrifuged at 201 rcf for 5 min.

    Article Title: Caldendrin represses neurite regeneration and growth in dorsal root ganglion neurons
    Article Snippet: DRGs (20–30) were harvested in NeurobasalTM media (Cat#21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin–EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), (Cat #17100, ThermoFisher) for 40 min. After the enzyme incubation, 10% horse serum was added to inactivate the proteases, the cell suspension was centrifuged at 500 rcf for 5 min.

    Article Title: NeuriteNet: A convolutional neural network for assessing morphological parameters of neurite growth
    Article Snippet: Lumbar DRGs were harvested into NeurobasalTM media (Cat #21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), Cat #17100, ThermoFisher) for 40 min. After addition of 10% FBS to inactivate the proteases, the cell suspension was centrifuged at 168 × g for 5 min.

    Article Title: Caldendrin represses neurite regeneration via a sex-dependent mechanism in sensory neurons
    Article Snippet: 20-30 DRGs were harvested into NeurobasalTM media (Cat#21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (-/-)), (Cat #17100, ThermoFisher) for 40 min. After the enzyme incubation, 10% horse serum was added to inactivate the proteases, the cell suspension was centrifuged at 500 rcf for 5 min.

    Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
    Article Snippet: Either HBSS with Calcium (14025092, Thermo Fisher Scientific Inc., Waltham, MA, USA) or without Calcium (14175095, Thermo Fisher Scientific Inc., Waltham, MA, USA) were used in the respective experiments, further supplemented with 2mM CaCl 2 or with 2 mM EGTA.

    Membrane:

    Article Title: Fasciola hepatica products can alter the response of bovine immune cells to Mycobacterium avium subsp. paratuberculosis
    Article Snippet: Following euthanasia by intra‐jugular administration of pentobarbital sodium (Euthanal 200 mg/mL, Merial Animal Health), lymph nodes were extracted during post‐mortem examination and suspended in ice‐cold Hank’s balanced salt solution (HBSS; without calcium or magnesium) supplemented with 1% antibiotic–antimycotic solution (Thermo Fisher Scientific).

    Article Title: STING signaling sensing of DRP1-dependent mtDNA release in kupffer cells contributes to lipopolysaccharide-induced liver injury in mice
    Article Snippet: A 26G catheter was inserted into the mouse portal vein, and the liver was perfused with 30 mL warmed Hank’s balanced salt solution (HBSS, without calcium or magnesium, GIBCO) containing 1% penicillin streptomycin to flush out the blood, followed by 40 mL DMEM/F12 (GBICO) containing 0.05% collagenase IV (GBICO) and 1% penicillin streptomycin.

    Article Title: NeuriteNet: A Convolutional Neural Network for determining morphological differences in neurite growth
    Article Snippet: Lumbar DRGs were harvested into NeurobasalTM media (Cat #21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), Cat #17100, ThermoFisher) for 40 min. After addition of 10% FBS to inactivate the proteases, the cell suspension was centrifuged at 201 rcf for 5 min.

    Article Title: Caldendrin represses neurite regeneration and growth in dorsal root ganglion neurons
    Article Snippet: DRGs (20–30) were harvested in NeurobasalTM media (Cat#21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin–EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), (Cat #17100, ThermoFisher) for 40 min. After the enzyme incubation, 10% horse serum was added to inactivate the proteases, the cell suspension was centrifuged at 500 rcf for 5 min.

    Article Title: NeuriteNet: A convolutional neural network for assessing morphological parameters of neurite growth
    Article Snippet: Lumbar DRGs were harvested into NeurobasalTM media (Cat #21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), Cat #17100, ThermoFisher) for 40 min. After addition of 10% FBS to inactivate the proteases, the cell suspension was centrifuged at 168 × g for 5 min.

    Article Title: Caldendrin represses neurite regeneration via a sex-dependent mechanism in sensory neurons
    Article Snippet: 20-30 DRGs were harvested into NeurobasalTM media (Cat#21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (-/-)), (Cat #17100, ThermoFisher) for 40 min. After the enzyme incubation, 10% horse serum was added to inactivate the proteases, the cell suspension was centrifuged at 500 rcf for 5 min.

    Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
    Article Snippet: Either HBSS with Calcium (14025092, Thermo Fisher Scientific Inc., Waltham, MA, USA) or without Calcium (14175095, Thermo Fisher Scientific Inc., Waltham, MA, USA) were used in the respective experiments, further supplemented with 2mM CaCl 2 or with 2 mM EGTA.

    Electron Microscopy:

    Article Title: Fasciola hepatica products can alter the response of bovine immune cells to Mycobacterium avium subsp. paratuberculosis
    Article Snippet: Following euthanasia by intra‐jugular administration of pentobarbital sodium (Euthanal 200 mg/mL, Merial Animal Health), lymph nodes were extracted during post‐mortem examination and suspended in ice‐cold Hank’s balanced salt solution (HBSS; without calcium or magnesium) supplemented with 1% antibiotic–antimycotic solution (Thermo Fisher Scientific).

    Article Title: STING signaling sensing of DRP1-dependent mtDNA release in kupffer cells contributes to lipopolysaccharide-induced liver injury in mice
    Article Snippet: A 26G catheter was inserted into the mouse portal vein, and the liver was perfused with 30 mL warmed Hank’s balanced salt solution (HBSS, without calcium or magnesium, GIBCO) containing 1% penicillin streptomycin to flush out the blood, followed by 40 mL DMEM/F12 (GBICO) containing 0.05% collagenase IV (GBICO) and 1% penicillin streptomycin.

    Article Title: NeuriteNet: A Convolutional Neural Network for determining morphological differences in neurite growth
    Article Snippet: Lumbar DRGs were harvested into NeurobasalTM media (Cat #21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), Cat #17100, ThermoFisher) for 40 min. After addition of 10% FBS to inactivate the proteases, the cell suspension was centrifuged at 201 rcf for 5 min.

    Article Title: Caldendrin represses neurite regeneration and growth in dorsal root ganglion neurons
    Article Snippet: DRGs (20–30) were harvested in NeurobasalTM media (Cat#21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin–EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), (Cat #17100, ThermoFisher) for 40 min. After the enzyme incubation, 10% horse serum was added to inactivate the proteases, the cell suspension was centrifuged at 500 rcf for 5 min.

    Article Title: NeuriteNet: A convolutional neural network for assessing morphological parameters of neurite growth
    Article Snippet: Lumbar DRGs were harvested into NeurobasalTM media (Cat #21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), Cat #17100, ThermoFisher) for 40 min. After addition of 10% FBS to inactivate the proteases, the cell suspension was centrifuged at 168 × g for 5 min.

    Article Title: Caldendrin represses neurite regeneration via a sex-dependent mechanism in sensory neurons
    Article Snippet: 20-30 DRGs were harvested into NeurobasalTM media (Cat#21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (-/-)), (Cat #17100, ThermoFisher) for 40 min. After the enzyme incubation, 10% horse serum was added to inactivate the proteases, the cell suspension was centrifuged at 500 rcf for 5 min.

    Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
    Article Snippet: Either HBSS with Calcium (14025092, Thermo Fisher Scientific Inc., Waltham, MA, USA) or without Calcium (14175095, Thermo Fisher Scientific Inc., Waltham, MA, USA) were used in the respective experiments, further supplemented with 2mM CaCl 2 or with 2 mM EGTA.

    Saline:

    Article Title: Fasciola hepatica products can alter the response of bovine immune cells to Mycobacterium avium subsp. paratuberculosis
    Article Snippet: Following euthanasia by intra‐jugular administration of pentobarbital sodium (Euthanal 200 mg/mL, Merial Animal Health), lymph nodes were extracted during post‐mortem examination and suspended in ice‐cold Hank’s balanced salt solution (HBSS; without calcium or magnesium) supplemented with 1% antibiotic–antimycotic solution (Thermo Fisher Scientific).

    Article Title: STING signaling sensing of DRP1-dependent mtDNA release in kupffer cells contributes to lipopolysaccharide-induced liver injury in mice
    Article Snippet: A 26G catheter was inserted into the mouse portal vein, and the liver was perfused with 30 mL warmed Hank’s balanced salt solution (HBSS, without calcium or magnesium, GIBCO) containing 1% penicillin streptomycin to flush out the blood, followed by 40 mL DMEM/F12 (GBICO) containing 0.05% collagenase IV (GBICO) and 1% penicillin streptomycin.

    Article Title: NeuriteNet: A Convolutional Neural Network for determining morphological differences in neurite growth
    Article Snippet: Lumbar DRGs were harvested into NeurobasalTM media (Cat #21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), Cat #17100, ThermoFisher) for 40 min. After addition of 10% FBS to inactivate the proteases, the cell suspension was centrifuged at 201 rcf for 5 min.

    Article Title: Caldendrin represses neurite regeneration and growth in dorsal root ganglion neurons
    Article Snippet: DRGs (20–30) were harvested in NeurobasalTM media (Cat#21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin–EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), (Cat #17100, ThermoFisher) for 40 min. After the enzyme incubation, 10% horse serum was added to inactivate the proteases, the cell suspension was centrifuged at 500 rcf for 5 min.

    Article Title: NeuriteNet: A convolutional neural network for assessing morphological parameters of neurite growth
    Article Snippet: Lumbar DRGs were harvested into NeurobasalTM media (Cat #21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), Cat #17100, ThermoFisher) for 40 min. After addition of 10% FBS to inactivate the proteases, the cell suspension was centrifuged at 168 × g for 5 min.

    Article Title: Caldendrin represses neurite regeneration via a sex-dependent mechanism in sensory neurons
    Article Snippet: 20-30 DRGs were harvested into NeurobasalTM media (Cat#21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (-/-)), (Cat #17100, ThermoFisher) for 40 min. After the enzyme incubation, 10% horse serum was added to inactivate the proteases, the cell suspension was centrifuged at 500 rcf for 5 min.

    Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
    Article Snippet: Either HBSS with Calcium (14025092, Thermo Fisher Scientific Inc., Waltham, MA, USA) or without Calcium (14175095, Thermo Fisher Scientific Inc., Waltham, MA, USA) were used in the respective experiments, further supplemented with 2mM CaCl 2 or with 2 mM EGTA.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Fasciola hepatica products can alter the response of bovine immune cells to Mycobacterium avium subsp. paratuberculosis
    Article Snippet: Following euthanasia by intra‐jugular administration of pentobarbital sodium (Euthanal 200 mg/mL, Merial Animal Health), lymph nodes were extracted during post‐mortem examination and suspended in ice‐cold Hank’s balanced salt solution (HBSS; without calcium or magnesium) supplemented with 1% antibiotic–antimycotic solution (Thermo Fisher Scientific).

    Article Title: STING signaling sensing of DRP1-dependent mtDNA release in kupffer cells contributes to lipopolysaccharide-induced liver injury in mice
    Article Snippet: A 26G catheter was inserted into the mouse portal vein, and the liver was perfused with 30 mL warmed Hank’s balanced salt solution (HBSS, without calcium or magnesium, GIBCO) containing 1% penicillin streptomycin to flush out the blood, followed by 40 mL DMEM/F12 (GBICO) containing 0.05% collagenase IV (GBICO) and 1% penicillin streptomycin.

    Article Title: NeuriteNet: A Convolutional Neural Network for determining morphological differences in neurite growth
    Article Snippet: Lumbar DRGs were harvested into NeurobasalTM media (Cat #21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), Cat #17100, ThermoFisher) for 40 min. After addition of 10% FBS to inactivate the proteases, the cell suspension was centrifuged at 201 rcf for 5 min.

    Article Title: Caldendrin represses neurite regeneration and growth in dorsal root ganglion neurons
    Article Snippet: DRGs (20–30) were harvested in NeurobasalTM media (Cat#21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin–EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), (Cat #17100, ThermoFisher) for 40 min. After the enzyme incubation, 10% horse serum was added to inactivate the proteases, the cell suspension was centrifuged at 500 rcf for 5 min.

    Article Title: NeuriteNet: A convolutional neural network for assessing morphological parameters of neurite growth
    Article Snippet: Lumbar DRGs were harvested into NeurobasalTM media (Cat #21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), Cat #17100, ThermoFisher) for 40 min. After addition of 10% FBS to inactivate the proteases, the cell suspension was centrifuged at 168 × g for 5 min.

    Article Title: Caldendrin represses neurite regeneration via a sex-dependent mechanism in sensory neurons
    Article Snippet: 20-30 DRGs were harvested into NeurobasalTM media (Cat#21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (-/-)), (Cat #17100, ThermoFisher) for 40 min. After the enzyme incubation, 10% horse serum was added to inactivate the proteases, the cell suspension was centrifuged at 500 rcf for 5 min.

    Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
    Article Snippet: Either HBSS with Calcium (14025092, Thermo Fisher Scientific Inc., Waltham, MA, USA) or without Calcium (14175095, Thermo Fisher Scientific Inc., Waltham, MA, USA) were used in the respective experiments, further supplemented with 2mM CaCl 2 or with 2 mM EGTA.

    Bicinchoninic Acid Protein Assay:

    Article Title: Fasciola hepatica products can alter the response of bovine immune cells to Mycobacterium avium subsp. paratuberculosis
    Article Snippet: Following euthanasia by intra‐jugular administration of pentobarbital sodium (Euthanal 200 mg/mL, Merial Animal Health), lymph nodes were extracted during post‐mortem examination and suspended in ice‐cold Hank’s balanced salt solution (HBSS; without calcium or magnesium) supplemented with 1% antibiotic–antimycotic solution (Thermo Fisher Scientific).

    Article Title: STING signaling sensing of DRP1-dependent mtDNA release in kupffer cells contributes to lipopolysaccharide-induced liver injury in mice
    Article Snippet: A 26G catheter was inserted into the mouse portal vein, and the liver was perfused with 30 mL warmed Hank’s balanced salt solution (HBSS, without calcium or magnesium, GIBCO) containing 1% penicillin streptomycin to flush out the blood, followed by 40 mL DMEM/F12 (GBICO) containing 0.05% collagenase IV (GBICO) and 1% penicillin streptomycin.

    Article Title: NeuriteNet: A Convolutional Neural Network for determining morphological differences in neurite growth
    Article Snippet: Lumbar DRGs were harvested into NeurobasalTM media (Cat #21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), Cat #17100, ThermoFisher) for 40 min. After addition of 10% FBS to inactivate the proteases, the cell suspension was centrifuged at 201 rcf for 5 min.

    Article Title: Caldendrin represses neurite regeneration and growth in dorsal root ganglion neurons
    Article Snippet: DRGs (20–30) were harvested in NeurobasalTM media (Cat#21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin–EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), (Cat #17100, ThermoFisher) for 40 min. After the enzyme incubation, 10% horse serum was added to inactivate the proteases, the cell suspension was centrifuged at 500 rcf for 5 min.

    Article Title: NeuriteNet: A convolutional neural network for assessing morphological parameters of neurite growth
    Article Snippet: Lumbar DRGs were harvested into NeurobasalTM media (Cat #21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), Cat #17100, ThermoFisher) for 40 min. After addition of 10% FBS to inactivate the proteases, the cell suspension was centrifuged at 168 × g for 5 min.

    Article Title: Caldendrin represses neurite regeneration via a sex-dependent mechanism in sensory neurons
    Article Snippet: 20-30 DRGs were harvested into NeurobasalTM media (Cat#21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (-/-)), (Cat #17100, ThermoFisher) for 40 min. After the enzyme incubation, 10% horse serum was added to inactivate the proteases, the cell suspension was centrifuged at 500 rcf for 5 min.

    Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
    Article Snippet: Either HBSS with Calcium (14025092, Thermo Fisher Scientific Inc., Waltham, MA, USA) or without Calcium (14175095, Thermo Fisher Scientific Inc., Waltham, MA, USA) were used in the respective experiments, further supplemented with 2mM CaCl 2 or with 2 mM EGTA.

    Western Blot:

    Article Title: Fasciola hepatica products can alter the response of bovine immune cells to Mycobacterium avium subsp. paratuberculosis
    Article Snippet: Following euthanasia by intra‐jugular administration of pentobarbital sodium (Euthanal 200 mg/mL, Merial Animal Health), lymph nodes were extracted during post‐mortem examination and suspended in ice‐cold Hank’s balanced salt solution (HBSS; without calcium or magnesium) supplemented with 1% antibiotic–antimycotic solution (Thermo Fisher Scientific).

    Article Title: STING signaling sensing of DRP1-dependent mtDNA release in kupffer cells contributes to lipopolysaccharide-induced liver injury in mice
    Article Snippet: A 26G catheter was inserted into the mouse portal vein, and the liver was perfused with 30 mL warmed Hank’s balanced salt solution (HBSS, without calcium or magnesium, GIBCO) containing 1% penicillin streptomycin to flush out the blood, followed by 40 mL DMEM/F12 (GBICO) containing 0.05% collagenase IV (GBICO) and 1% penicillin streptomycin.

    Article Title: NeuriteNet: A Convolutional Neural Network for determining morphological differences in neurite growth
    Article Snippet: Lumbar DRGs were harvested into NeurobasalTM media (Cat #21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), Cat #17100, ThermoFisher) for 40 min. After addition of 10% FBS to inactivate the proteases, the cell suspension was centrifuged at 201 rcf for 5 min.

    Article Title: Caldendrin represses neurite regeneration and growth in dorsal root ganglion neurons
    Article Snippet: DRGs (20–30) were harvested in NeurobasalTM media (Cat#21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin–EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), (Cat #17100, ThermoFisher) for 40 min. After the enzyme incubation, 10% horse serum was added to inactivate the proteases, the cell suspension was centrifuged at 500 rcf for 5 min.

    Article Title: NeuriteNet: A convolutional neural network for assessing morphological parameters of neurite growth
    Article Snippet: Lumbar DRGs were harvested into NeurobasalTM media (Cat #21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), Cat #17100, ThermoFisher) for 40 min. After addition of 10% FBS to inactivate the proteases, the cell suspension was centrifuged at 168 × g for 5 min.

    Article Title: Caldendrin represses neurite regeneration via a sex-dependent mechanism in sensory neurons
    Article Snippet: 20-30 DRGs were harvested into NeurobasalTM media (Cat#21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (-/-)), (Cat #17100, ThermoFisher) for 40 min. After the enzyme incubation, 10% horse serum was added to inactivate the proteases, the cell suspension was centrifuged at 500 rcf for 5 min.

    Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
    Article Snippet: Either HBSS with Calcium (14025092, Thermo Fisher Scientific Inc., Waltham, MA, USA) or without Calcium (14175095, Thermo Fisher Scientific Inc., Waltham, MA, USA) were used in the respective experiments, further supplemented with 2mM CaCl 2 or with 2 mM EGTA.

    RNA Sequencing:

    Article Title: Fasciola hepatica products can alter the response of bovine immune cells to Mycobacterium avium subsp. paratuberculosis
    Article Snippet: Following euthanasia by intra‐jugular administration of pentobarbital sodium (Euthanal 200 mg/mL, Merial Animal Health), lymph nodes were extracted during post‐mortem examination and suspended in ice‐cold Hank’s balanced salt solution (HBSS; without calcium or magnesium) supplemented with 1% antibiotic–antimycotic solution (Thermo Fisher Scientific).

    Article Title: STING signaling sensing of DRP1-dependent mtDNA release in kupffer cells contributes to lipopolysaccharide-induced liver injury in mice
    Article Snippet: A 26G catheter was inserted into the mouse portal vein, and the liver was perfused with 30 mL warmed Hank’s balanced salt solution (HBSS, without calcium or magnesium, GIBCO) containing 1% penicillin streptomycin to flush out the blood, followed by 40 mL DMEM/F12 (GBICO) containing 0.05% collagenase IV (GBICO) and 1% penicillin streptomycin.

    Article Title: NeuriteNet: A Convolutional Neural Network for determining morphological differences in neurite growth
    Article Snippet: Lumbar DRGs were harvested into NeurobasalTM media (Cat #21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), Cat #17100, ThermoFisher) for 40 min. After addition of 10% FBS to inactivate the proteases, the cell suspension was centrifuged at 201 rcf for 5 min.

    Article Title: Caldendrin represses neurite regeneration and growth in dorsal root ganglion neurons
    Article Snippet: DRGs (20–30) were harvested in NeurobasalTM media (Cat#21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin–EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), (Cat #17100, ThermoFisher) for 40 min. After the enzyme incubation, 10% horse serum was added to inactivate the proteases, the cell suspension was centrifuged at 500 rcf for 5 min.

    Article Title: NeuriteNet: A convolutional neural network for assessing morphological parameters of neurite growth
    Article Snippet: Lumbar DRGs were harvested into NeurobasalTM media (Cat #21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), Cat #17100, ThermoFisher) for 40 min. After addition of 10% FBS to inactivate the proteases, the cell suspension was centrifuged at 168 × g for 5 min.

    Article Title: Caldendrin represses neurite regeneration via a sex-dependent mechanism in sensory neurons
    Article Snippet: 20-30 DRGs were harvested into NeurobasalTM media (Cat#21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (-/-)), (Cat #17100, ThermoFisher) for 40 min. After the enzyme incubation, 10% horse serum was added to inactivate the proteases, the cell suspension was centrifuged at 500 rcf for 5 min.

    Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
    Article Snippet: Either HBSS with Calcium (14025092, Thermo Fisher Scientific Inc., Waltham, MA, USA) or without Calcium (14175095, Thermo Fisher Scientific Inc., Waltham, MA, USA) were used in the respective experiments, further supplemented with 2mM CaCl 2 or with 2 mM EGTA.

    Gene Expression:

    Article Title: Fasciola hepatica products can alter the response of bovine immune cells to Mycobacterium avium subsp. paratuberculosis
    Article Snippet: Following euthanasia by intra‐jugular administration of pentobarbital sodium (Euthanal 200 mg/mL, Merial Animal Health), lymph nodes were extracted during post‐mortem examination and suspended in ice‐cold Hank’s balanced salt solution (HBSS; without calcium or magnesium) supplemented with 1% antibiotic–antimycotic solution (Thermo Fisher Scientific).

    Article Title: STING signaling sensing of DRP1-dependent mtDNA release in kupffer cells contributes to lipopolysaccharide-induced liver injury in mice
    Article Snippet: A 26G catheter was inserted into the mouse portal vein, and the liver was perfused with 30 mL warmed Hank’s balanced salt solution (HBSS, without calcium or magnesium, GIBCO) containing 1% penicillin streptomycin to flush out the blood, followed by 40 mL DMEM/F12 (GBICO) containing 0.05% collagenase IV (GBICO) and 1% penicillin streptomycin.

    Article Title: NeuriteNet: A Convolutional Neural Network for determining morphological differences in neurite growth
    Article Snippet: Lumbar DRGs were harvested into NeurobasalTM media (Cat #21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), Cat #17100, ThermoFisher) for 40 min. After addition of 10% FBS to inactivate the proteases, the cell suspension was centrifuged at 201 rcf for 5 min.

    Article Title: Caldendrin represses neurite regeneration and growth in dorsal root ganglion neurons
    Article Snippet: DRGs (20–30) were harvested in NeurobasalTM media (Cat#21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin–EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), (Cat #17100, ThermoFisher) for 40 min. After the enzyme incubation, 10% horse serum was added to inactivate the proteases, the cell suspension was centrifuged at 500 rcf for 5 min.

    Article Title: NeuriteNet: A convolutional neural network for assessing morphological parameters of neurite growth
    Article Snippet: Lumbar DRGs were harvested into NeurobasalTM media (Cat #21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), Cat #17100, ThermoFisher) for 40 min. After addition of 10% FBS to inactivate the proteases, the cell suspension was centrifuged at 168 × g for 5 min.

    Article Title: Caldendrin represses neurite regeneration via a sex-dependent mechanism in sensory neurons
    Article Snippet: 20-30 DRGs were harvested into NeurobasalTM media (Cat#21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (-/-)), (Cat #17100, ThermoFisher) for 40 min. After the enzyme incubation, 10% horse serum was added to inactivate the proteases, the cell suspension was centrifuged at 500 rcf for 5 min.

    Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
    Article Snippet: Either HBSS with Calcium (14025092, Thermo Fisher Scientific Inc., Waltham, MA, USA) or without Calcium (14175095, Thermo Fisher Scientific Inc., Waltham, MA, USA) were used in the respective experiments, further supplemented with 2mM CaCl 2 or with 2 mM EGTA.

    Imaging:

    Article Title: Fasciola hepatica products can alter the response of bovine immune cells to Mycobacterium avium subsp. paratuberculosis
    Article Snippet: Following euthanasia by intra‐jugular administration of pentobarbital sodium (Euthanal 200 mg/mL, Merial Animal Health), lymph nodes were extracted during post‐mortem examination and suspended in ice‐cold Hank’s balanced salt solution (HBSS; without calcium or magnesium) supplemented with 1% antibiotic–antimycotic solution (Thermo Fisher Scientific).

    Article Title: STING signaling sensing of DRP1-dependent mtDNA release in kupffer cells contributes to lipopolysaccharide-induced liver injury in mice
    Article Snippet: A 26G catheter was inserted into the mouse portal vein, and the liver was perfused with 30 mL warmed Hank’s balanced salt solution (HBSS, without calcium or magnesium, GIBCO) containing 1% penicillin streptomycin to flush out the blood, followed by 40 mL DMEM/F12 (GBICO) containing 0.05% collagenase IV (GBICO) and 1% penicillin streptomycin.

    Article Title: NeuriteNet: A Convolutional Neural Network for determining morphological differences in neurite growth
    Article Snippet: Lumbar DRGs were harvested into NeurobasalTM media (Cat #21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), Cat #17100, ThermoFisher) for 40 min. After addition of 10% FBS to inactivate the proteases, the cell suspension was centrifuged at 201 rcf for 5 min.

    Article Title: Caldendrin represses neurite regeneration and growth in dorsal root ganglion neurons
    Article Snippet: DRGs (20–30) were harvested in NeurobasalTM media (Cat#21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin–EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), (Cat #17100, ThermoFisher) for 40 min. After the enzyme incubation, 10% horse serum was added to inactivate the proteases, the cell suspension was centrifuged at 500 rcf for 5 min.

    Article Title: NeuriteNet: A convolutional neural network for assessing morphological parameters of neurite growth
    Article Snippet: Lumbar DRGs were harvested into NeurobasalTM media (Cat #21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), Cat #17100, ThermoFisher) for 40 min. After addition of 10% FBS to inactivate the proteases, the cell suspension was centrifuged at 168 × g for 5 min.

    Article Title: Caldendrin represses neurite regeneration via a sex-dependent mechanism in sensory neurons
    Article Snippet: 20-30 DRGs were harvested into NeurobasalTM media (Cat#21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (-/-)), (Cat #17100, ThermoFisher) for 40 min. After the enzyme incubation, 10% horse serum was added to inactivate the proteases, the cell suspension was centrifuged at 500 rcf for 5 min.

    Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
    Article Snippet: Either HBSS with Calcium (14025092, Thermo Fisher Scientific Inc., Waltham, MA, USA) or without Calcium (14175095, Thermo Fisher Scientific Inc., Waltham, MA, USA) were used in the respective experiments, further supplemented with 2mM CaCl 2 or with 2 mM EGTA.

    Plasmid Preparation:

    Article Title: Fasciola hepatica products can alter the response of bovine immune cells to Mycobacterium avium subsp. paratuberculosis
    Article Snippet: Following euthanasia by intra‐jugular administration of pentobarbital sodium (Euthanal 200 mg/mL, Merial Animal Health), lymph nodes were extracted during post‐mortem examination and suspended in ice‐cold Hank’s balanced salt solution (HBSS; without calcium or magnesium) supplemented with 1% antibiotic–antimycotic solution (Thermo Fisher Scientific).

    Article Title: STING signaling sensing of DRP1-dependent mtDNA release in kupffer cells contributes to lipopolysaccharide-induced liver injury in mice
    Article Snippet: A 26G catheter was inserted into the mouse portal vein, and the liver was perfused with 30 mL warmed Hank’s balanced salt solution (HBSS, without calcium or magnesium, GIBCO) containing 1% penicillin streptomycin to flush out the blood, followed by 40 mL DMEM/F12 (GBICO) containing 0.05% collagenase IV (GBICO) and 1% penicillin streptomycin.

    Article Title: NeuriteNet: A Convolutional Neural Network for determining morphological differences in neurite growth
    Article Snippet: Lumbar DRGs were harvested into NeurobasalTM media (Cat #21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), Cat #17100, ThermoFisher) for 40 min. After addition of 10% FBS to inactivate the proteases, the cell suspension was centrifuged at 201 rcf for 5 min.

    Article Title: Caldendrin represses neurite regeneration and growth in dorsal root ganglion neurons
    Article Snippet: DRGs (20–30) were harvested in NeurobasalTM media (Cat#21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin–EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), (Cat #17100, ThermoFisher) for 40 min. After the enzyme incubation, 10% horse serum was added to inactivate the proteases, the cell suspension was centrifuged at 500 rcf for 5 min.

    Article Title: NeuriteNet: A convolutional neural network for assessing morphological parameters of neurite growth
    Article Snippet: Lumbar DRGs were harvested into NeurobasalTM media (Cat #21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), Cat #17100, ThermoFisher) for 40 min. After addition of 10% FBS to inactivate the proteases, the cell suspension was centrifuged at 168 × g for 5 min.

    Article Title: Caldendrin represses neurite regeneration via a sex-dependent mechanism in sensory neurons
    Article Snippet: 20-30 DRGs were harvested into NeurobasalTM media (Cat#21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (-/-)), (Cat #17100, ThermoFisher) for 40 min. After the enzyme incubation, 10% horse serum was added to inactivate the proteases, the cell suspension was centrifuged at 500 rcf for 5 min.

    Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
    Article Snippet: Either HBSS with Calcium (14025092, Thermo Fisher Scientific Inc., Waltham, MA, USA) or without Calcium (14175095, Thermo Fisher Scientific Inc., Waltham, MA, USA) were used in the respective experiments, further supplemented with 2mM CaCl 2 or with 2 mM EGTA.

    shRNA:

    Article Title: Fasciola hepatica products can alter the response of bovine immune cells to Mycobacterium avium subsp. paratuberculosis
    Article Snippet: Following euthanasia by intra‐jugular administration of pentobarbital sodium (Euthanal 200 mg/mL, Merial Animal Health), lymph nodes were extracted during post‐mortem examination and suspended in ice‐cold Hank’s balanced salt solution (HBSS; without calcium or magnesium) supplemented with 1% antibiotic–antimycotic solution (Thermo Fisher Scientific).

    Article Title: STING signaling sensing of DRP1-dependent mtDNA release in kupffer cells contributes to lipopolysaccharide-induced liver injury in mice
    Article Snippet: A 26G catheter was inserted into the mouse portal vein, and the liver was perfused with 30 mL warmed Hank’s balanced salt solution (HBSS, without calcium or magnesium, GIBCO) containing 1% penicillin streptomycin to flush out the blood, followed by 40 mL DMEM/F12 (GBICO) containing 0.05% collagenase IV (GBICO) and 1% penicillin streptomycin.

    Article Title: NeuriteNet: A Convolutional Neural Network for determining morphological differences in neurite growth
    Article Snippet: Lumbar DRGs were harvested into NeurobasalTM media (Cat #21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), Cat #17100, ThermoFisher) for 40 min. After addition of 10% FBS to inactivate the proteases, the cell suspension was centrifuged at 201 rcf for 5 min.

    Article Title: Caldendrin represses neurite regeneration and growth in dorsal root ganglion neurons
    Article Snippet: DRGs (20–30) were harvested in NeurobasalTM media (Cat#21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin–EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), (Cat #17100, ThermoFisher) for 40 min. After the enzyme incubation, 10% horse serum was added to inactivate the proteases, the cell suspension was centrifuged at 500 rcf for 5 min.

    Article Title: NeuriteNet: A convolutional neural network for assessing morphological parameters of neurite growth
    Article Snippet: Lumbar DRGs were harvested into NeurobasalTM media (Cat #21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), Cat #17100, ThermoFisher) for 40 min. After addition of 10% FBS to inactivate the proteases, the cell suspension was centrifuged at 168 × g for 5 min.

    Article Title: Caldendrin represses neurite regeneration via a sex-dependent mechanism in sensory neurons
    Article Snippet: 20-30 DRGs were harvested into NeurobasalTM media (Cat#21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (-/-)), (Cat #17100, ThermoFisher) for 40 min. After the enzyme incubation, 10% horse serum was added to inactivate the proteases, the cell suspension was centrifuged at 500 rcf for 5 min.

    Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
    Article Snippet: Either HBSS with Calcium (14025092, Thermo Fisher Scientific Inc., Waltham, MA, USA) or without Calcium (14175095, Thermo Fisher Scientific Inc., Waltham, MA, USA) were used in the respective experiments, further supplemented with 2mM CaCl 2 or with 2 mM EGTA.

    Control:

    Article Title: Fasciola hepatica products can alter the response of bovine immune cells to Mycobacterium avium subsp. paratuberculosis
    Article Snippet: Following euthanasia by intra‐jugular administration of pentobarbital sodium (Euthanal 200 mg/mL, Merial Animal Health), lymph nodes were extracted during post‐mortem examination and suspended in ice‐cold Hank’s balanced salt solution (HBSS; without calcium or magnesium) supplemented with 1% antibiotic–antimycotic solution (Thermo Fisher Scientific).

    Article Title: STING signaling sensing of DRP1-dependent mtDNA release in kupffer cells contributes to lipopolysaccharide-induced liver injury in mice
    Article Snippet: A 26G catheter was inserted into the mouse portal vein, and the liver was perfused with 30 mL warmed Hank’s balanced salt solution (HBSS, without calcium or magnesium, GIBCO) containing 1% penicillin streptomycin to flush out the blood, followed by 40 mL DMEM/F12 (GBICO) containing 0.05% collagenase IV (GBICO) and 1% penicillin streptomycin.

    Article Title: NeuriteNet: A Convolutional Neural Network for determining morphological differences in neurite growth
    Article Snippet: Lumbar DRGs were harvested into NeurobasalTM media (Cat #21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), Cat #17100, ThermoFisher) for 40 min. After addition of 10% FBS to inactivate the proteases, the cell suspension was centrifuged at 201 rcf for 5 min.

    Article Title: Caldendrin represses neurite regeneration and growth in dorsal root ganglion neurons
    Article Snippet: DRGs (20–30) were harvested in NeurobasalTM media (Cat#21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin–EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), (Cat #17100, ThermoFisher) for 40 min. After the enzyme incubation, 10% horse serum was added to inactivate the proteases, the cell suspension was centrifuged at 500 rcf for 5 min.

    Article Title: NeuriteNet: A convolutional neural network for assessing morphological parameters of neurite growth
    Article Snippet: Lumbar DRGs were harvested into NeurobasalTM media (Cat #21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), Cat #17100, ThermoFisher) for 40 min. After addition of 10% FBS to inactivate the proteases, the cell suspension was centrifuged at 168 × g for 5 min.

    Article Title: Caldendrin represses neurite regeneration via a sex-dependent mechanism in sensory neurons
    Article Snippet: 20-30 DRGs were harvested into NeurobasalTM media (Cat#21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (-/-)), (Cat #17100, ThermoFisher) for 40 min. After the enzyme incubation, 10% horse serum was added to inactivate the proteases, the cell suspension was centrifuged at 500 rcf for 5 min.

    Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
    Article Snippet: Either HBSS with Calcium (14025092, Thermo Fisher Scientific Inc., Waltham, MA, USA) or without Calcium (14175095, Thermo Fisher Scientific Inc., Waltham, MA, USA) were used in the respective experiments, further supplemented with 2mM CaCl 2 or with 2 mM EGTA.

    Luciferase:

    Article Title: Fasciola hepatica products can alter the response of bovine immune cells to Mycobacterium avium subsp. paratuberculosis
    Article Snippet: Following euthanasia by intra‐jugular administration of pentobarbital sodium (Euthanal 200 mg/mL, Merial Animal Health), lymph nodes were extracted during post‐mortem examination and suspended in ice‐cold Hank’s balanced salt solution (HBSS; without calcium or magnesium) supplemented with 1% antibiotic–antimycotic solution (Thermo Fisher Scientific).

    Article Title: STING signaling sensing of DRP1-dependent mtDNA release in kupffer cells contributes to lipopolysaccharide-induced liver injury in mice
    Article Snippet: A 26G catheter was inserted into the mouse portal vein, and the liver was perfused with 30 mL warmed Hank’s balanced salt solution (HBSS, without calcium or magnesium, GIBCO) containing 1% penicillin streptomycin to flush out the blood, followed by 40 mL DMEM/F12 (GBICO) containing 0.05% collagenase IV (GBICO) and 1% penicillin streptomycin.

    Article Title: NeuriteNet: A Convolutional Neural Network for determining morphological differences in neurite growth
    Article Snippet: Lumbar DRGs were harvested into NeurobasalTM media (Cat #21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), Cat #17100, ThermoFisher) for 40 min. After addition of 10% FBS to inactivate the proteases, the cell suspension was centrifuged at 201 rcf for 5 min.

    Article Title: Caldendrin represses neurite regeneration and growth in dorsal root ganglion neurons
    Article Snippet: DRGs (20–30) were harvested in NeurobasalTM media (Cat#21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin–EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), (Cat #17100, ThermoFisher) for 40 min. After the enzyme incubation, 10% horse serum was added to inactivate the proteases, the cell suspension was centrifuged at 500 rcf for 5 min.

    Article Title: NeuriteNet: A convolutional neural network for assessing morphological parameters of neurite growth
    Article Snippet: Lumbar DRGs were harvested into NeurobasalTM media (Cat #21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), Cat #17100, ThermoFisher) for 40 min. After addition of 10% FBS to inactivate the proteases, the cell suspension was centrifuged at 168 × g for 5 min.

    Article Title: Caldendrin represses neurite regeneration via a sex-dependent mechanism in sensory neurons
    Article Snippet: 20-30 DRGs were harvested into NeurobasalTM media (Cat#21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (-/-)), (Cat #17100, ThermoFisher) for 40 min. After the enzyme incubation, 10% horse serum was added to inactivate the proteases, the cell suspension was centrifuged at 500 rcf for 5 min.

    Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
    Article Snippet: Either HBSS with Calcium (14025092, Thermo Fisher Scientific Inc., Waltham, MA, USA) or without Calcium (14175095, Thermo Fisher Scientific Inc., Waltham, MA, USA) were used in the respective experiments, further supplemented with 2mM CaCl 2 or with 2 mM EGTA.

    Cloning:

    Article Title: Fasciola hepatica products can alter the response of bovine immune cells to Mycobacterium avium subsp. paratuberculosis
    Article Snippet: Following euthanasia by intra‐jugular administration of pentobarbital sodium (Euthanal 200 mg/mL, Merial Animal Health), lymph nodes were extracted during post‐mortem examination and suspended in ice‐cold Hank’s balanced salt solution (HBSS; without calcium or magnesium) supplemented with 1% antibiotic–antimycotic solution (Thermo Fisher Scientific).

    Article Title: STING signaling sensing of DRP1-dependent mtDNA release in kupffer cells contributes to lipopolysaccharide-induced liver injury in mice
    Article Snippet: A 26G catheter was inserted into the mouse portal vein, and the liver was perfused with 30 mL warmed Hank’s balanced salt solution (HBSS, without calcium or magnesium, GIBCO) containing 1% penicillin streptomycin to flush out the blood, followed by 40 mL DMEM/F12 (GBICO) containing 0.05% collagenase IV (GBICO) and 1% penicillin streptomycin.

    Article Title: NeuriteNet: A Convolutional Neural Network for determining morphological differences in neurite growth
    Article Snippet: Lumbar DRGs were harvested into NeurobasalTM media (Cat #21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), Cat #17100, ThermoFisher) for 40 min. After addition of 10% FBS to inactivate the proteases, the cell suspension was centrifuged at 201 rcf for 5 min.

    Article Title: Caldendrin represses neurite regeneration and growth in dorsal root ganglion neurons
    Article Snippet: DRGs (20–30) were harvested in NeurobasalTM media (Cat#21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin–EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), (Cat #17100, ThermoFisher) for 40 min. After the enzyme incubation, 10% horse serum was added to inactivate the proteases, the cell suspension was centrifuged at 500 rcf for 5 min.

    Article Title: NeuriteNet: A convolutional neural network for assessing morphological parameters of neurite growth
    Article Snippet: Lumbar DRGs were harvested into NeurobasalTM media (Cat #21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), Cat #17100, ThermoFisher) for 40 min. After addition of 10% FBS to inactivate the proteases, the cell suspension was centrifuged at 168 × g for 5 min.

    Article Title: Caldendrin represses neurite regeneration via a sex-dependent mechanism in sensory neurons
    Article Snippet: 20-30 DRGs were harvested into NeurobasalTM media (Cat#21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (-/-)), (Cat #17100, ThermoFisher) for 40 min. After the enzyme incubation, 10% horse serum was added to inactivate the proteases, the cell suspension was centrifuged at 500 rcf for 5 min.

    Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
    Article Snippet: Either HBSS with Calcium (14025092, Thermo Fisher Scientific Inc., Waltham, MA, USA) or without Calcium (14175095, Thermo Fisher Scientific Inc., Waltham, MA, USA) were used in the respective experiments, further supplemented with 2mM CaCl 2 or with 2 mM EGTA.

    Expressing:

    Article Title: Fasciola hepatica products can alter the response of bovine immune cells to Mycobacterium avium subsp. paratuberculosis
    Article Snippet: Following euthanasia by intra‐jugular administration of pentobarbital sodium (Euthanal 200 mg/mL, Merial Animal Health), lymph nodes were extracted during post‐mortem examination and suspended in ice‐cold Hank’s balanced salt solution (HBSS; without calcium or magnesium) supplemented with 1% antibiotic–antimycotic solution (Thermo Fisher Scientific).

    Article Title: STING signaling sensing of DRP1-dependent mtDNA release in kupffer cells contributes to lipopolysaccharide-induced liver injury in mice
    Article Snippet: A 26G catheter was inserted into the mouse portal vein, and the liver was perfused with 30 mL warmed Hank’s balanced salt solution (HBSS, without calcium or magnesium, GIBCO) containing 1% penicillin streptomycin to flush out the blood, followed by 40 mL DMEM/F12 (GBICO) containing 0.05% collagenase IV (GBICO) and 1% penicillin streptomycin.

    Article Title: NeuriteNet: A Convolutional Neural Network for determining morphological differences in neurite growth
    Article Snippet: Lumbar DRGs were harvested into NeurobasalTM media (Cat #21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), Cat #17100, ThermoFisher) for 40 min. After addition of 10% FBS to inactivate the proteases, the cell suspension was centrifuged at 201 rcf for 5 min.

    Article Title: Caldendrin represses neurite regeneration and growth in dorsal root ganglion neurons
    Article Snippet: DRGs (20–30) were harvested in NeurobasalTM media (Cat#21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin–EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), (Cat #17100, ThermoFisher) for 40 min. After the enzyme incubation, 10% horse serum was added to inactivate the proteases, the cell suspension was centrifuged at 500 rcf for 5 min.

    Article Title: NeuriteNet: A convolutional neural network for assessing morphological parameters of neurite growth
    Article Snippet: Lumbar DRGs were harvested into NeurobasalTM media (Cat #21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), Cat #17100, ThermoFisher) for 40 min. After addition of 10% FBS to inactivate the proteases, the cell suspension was centrifuged at 168 × g for 5 min.

    Article Title: Caldendrin represses neurite regeneration via a sex-dependent mechanism in sensory neurons
    Article Snippet: 20-30 DRGs were harvested into NeurobasalTM media (Cat#21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (-/-)), (Cat #17100, ThermoFisher) for 40 min. After the enzyme incubation, 10% horse serum was added to inactivate the proteases, the cell suspension was centrifuged at 500 rcf for 5 min.

    Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
    Article Snippet: Either HBSS with Calcium (14025092, Thermo Fisher Scientific Inc., Waltham, MA, USA) or without Calcium (14175095, Thermo Fisher Scientific Inc., Waltham, MA, USA) were used in the respective experiments, further supplemented with 2mM CaCl 2 or with 2 mM EGTA.

    Software:

    Article Title: Fasciola hepatica products can alter the response of bovine immune cells to Mycobacterium avium subsp. paratuberculosis
    Article Snippet: Following euthanasia by intra‐jugular administration of pentobarbital sodium (Euthanal 200 mg/mL, Merial Animal Health), lymph nodes were extracted during post‐mortem examination and suspended in ice‐cold Hank’s balanced salt solution (HBSS; without calcium or magnesium) supplemented with 1% antibiotic–antimycotic solution (Thermo Fisher Scientific).

    Article Title: STING signaling sensing of DRP1-dependent mtDNA release in kupffer cells contributes to lipopolysaccharide-induced liver injury in mice
    Article Snippet: A 26G catheter was inserted into the mouse portal vein, and the liver was perfused with 30 mL warmed Hank’s balanced salt solution (HBSS, without calcium or magnesium, GIBCO) containing 1% penicillin streptomycin to flush out the blood, followed by 40 mL DMEM/F12 (GBICO) containing 0.05% collagenase IV (GBICO) and 1% penicillin streptomycin.

    Article Title: NeuriteNet: A Convolutional Neural Network for determining morphological differences in neurite growth
    Article Snippet: Lumbar DRGs were harvested into NeurobasalTM media (Cat #21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), Cat #17100, ThermoFisher) for 40 min. After addition of 10% FBS to inactivate the proteases, the cell suspension was centrifuged at 201 rcf for 5 min.

    Article Title: Caldendrin represses neurite regeneration and growth in dorsal root ganglion neurons
    Article Snippet: DRGs (20–30) were harvested in NeurobasalTM media (Cat#21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin–EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), (Cat #17100, ThermoFisher) for 40 min. After the enzyme incubation, 10% horse serum was added to inactivate the proteases, the cell suspension was centrifuged at 500 rcf for 5 min.

    Article Title: NeuriteNet: A convolutional neural network for assessing morphological parameters of neurite growth
    Article Snippet: Lumbar DRGs were harvested into NeurobasalTM media (Cat #21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), Cat #17100, ThermoFisher) for 40 min. After addition of 10% FBS to inactivate the proteases, the cell suspension was centrifuged at 168 × g for 5 min.

    Article Title: Caldendrin represses neurite regeneration via a sex-dependent mechanism in sensory neurons
    Article Snippet: 20-30 DRGs were harvested into NeurobasalTM media (Cat#21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (-/-)), (Cat #17100, ThermoFisher) for 40 min. After the enzyme incubation, 10% horse serum was added to inactivate the proteases, the cell suspension was centrifuged at 500 rcf for 5 min.

    Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
    Article Snippet: Either HBSS with Calcium (14025092, Thermo Fisher Scientific Inc., Waltham, MA, USA) or without Calcium (14175095, Thermo Fisher Scientific Inc., Waltham, MA, USA) were used in the respective experiments, further supplemented with 2mM CaCl 2 or with 2 mM EGTA.

    Cell Analysis:

    Article Title: Fasciola hepatica products can alter the response of bovine immune cells to Mycobacterium avium subsp. paratuberculosis
    Article Snippet: Following euthanasia by intra‐jugular administration of pentobarbital sodium (Euthanal 200 mg/mL, Merial Animal Health), lymph nodes were extracted during post‐mortem examination and suspended in ice‐cold Hank’s balanced salt solution (HBSS; without calcium or magnesium) supplemented with 1% antibiotic–antimycotic solution (Thermo Fisher Scientific).

    Article Title: STING signaling sensing of DRP1-dependent mtDNA release in kupffer cells contributes to lipopolysaccharide-induced liver injury in mice
    Article Snippet: A 26G catheter was inserted into the mouse portal vein, and the liver was perfused with 30 mL warmed Hank’s balanced salt solution (HBSS, without calcium or magnesium, GIBCO) containing 1% penicillin streptomycin to flush out the blood, followed by 40 mL DMEM/F12 (GBICO) containing 0.05% collagenase IV (GBICO) and 1% penicillin streptomycin.

    Article Title: NeuriteNet: A Convolutional Neural Network for determining morphological differences in neurite growth
    Article Snippet: Lumbar DRGs were harvested into NeurobasalTM media (Cat #21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), Cat #17100, ThermoFisher) for 40 min. After addition of 10% FBS to inactivate the proteases, the cell suspension was centrifuged at 201 rcf for 5 min.

    Article Title: Caldendrin represses neurite regeneration and growth in dorsal root ganglion neurons
    Article Snippet: DRGs (20–30) were harvested in NeurobasalTM media (Cat#21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin–EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), (Cat #17100, ThermoFisher) for 40 min. After the enzyme incubation, 10% horse serum was added to inactivate the proteases, the cell suspension was centrifuged at 500 rcf for 5 min.

    Article Title: NeuriteNet: A convolutional neural network for assessing morphological parameters of neurite growth
    Article Snippet: Lumbar DRGs were harvested into NeurobasalTM media (Cat #21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), Cat #17100, ThermoFisher) for 40 min. After addition of 10% FBS to inactivate the proteases, the cell suspension was centrifuged at 168 × g for 5 min.

    Article Title: Caldendrin represses neurite regeneration via a sex-dependent mechanism in sensory neurons
    Article Snippet: 20-30 DRGs were harvested into NeurobasalTM media (Cat#21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (-/-)), (Cat #17100, ThermoFisher) for 40 min. After the enzyme incubation, 10% horse serum was added to inactivate the proteases, the cell suspension was centrifuged at 500 rcf for 5 min.

    Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
    Article Snippet: Either HBSS with Calcium (14025092, Thermo Fisher Scientific Inc., Waltham, MA, USA) or without Calcium (14175095, Thermo Fisher Scientific Inc., Waltham, MA, USA) were used in the respective experiments, further supplemented with 2mM CaCl 2 or with 2 mM EGTA.

    Microscopy:

    Article Title: Fasciola hepatica products can alter the response of bovine immune cells to Mycobacterium avium subsp. paratuberculosis
    Article Snippet: Following euthanasia by intra‐jugular administration of pentobarbital sodium (Euthanal 200 mg/mL, Merial Animal Health), lymph nodes were extracted during post‐mortem examination and suspended in ice‐cold Hank’s balanced salt solution (HBSS; without calcium or magnesium) supplemented with 1% antibiotic–antimycotic solution (Thermo Fisher Scientific).

    Article Title: STING signaling sensing of DRP1-dependent mtDNA release in kupffer cells contributes to lipopolysaccharide-induced liver injury in mice
    Article Snippet: A 26G catheter was inserted into the mouse portal vein, and the liver was perfused with 30 mL warmed Hank’s balanced salt solution (HBSS, without calcium or magnesium, GIBCO) containing 1% penicillin streptomycin to flush out the blood, followed by 40 mL DMEM/F12 (GBICO) containing 0.05% collagenase IV (GBICO) and 1% penicillin streptomycin.

    Article Title: NeuriteNet: A Convolutional Neural Network for determining morphological differences in neurite growth
    Article Snippet: Lumbar DRGs were harvested into NeurobasalTM media (Cat #21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), Cat #17100, ThermoFisher) for 40 min. After addition of 10% FBS to inactivate the proteases, the cell suspension was centrifuged at 201 rcf for 5 min.

    Article Title: Caldendrin represses neurite regeneration and growth in dorsal root ganglion neurons
    Article Snippet: DRGs (20–30) were harvested in NeurobasalTM media (Cat#21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin–EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), (Cat #17100, ThermoFisher) for 40 min. After the enzyme incubation, 10% horse serum was added to inactivate the proteases, the cell suspension was centrifuged at 500 rcf for 5 min.

    Article Title: NeuriteNet: A convolutional neural network for assessing morphological parameters of neurite growth
    Article Snippet: Lumbar DRGs were harvested into NeurobasalTM media (Cat #21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), Cat #17100, ThermoFisher) for 40 min. After addition of 10% FBS to inactivate the proteases, the cell suspension was centrifuged at 168 × g for 5 min.

    Article Title: Caldendrin represses neurite regeneration via a sex-dependent mechanism in sensory neurons
    Article Snippet: 20-30 DRGs were harvested into NeurobasalTM media (Cat#21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (-/-)), (Cat #17100, ThermoFisher) for 40 min. After the enzyme incubation, 10% horse serum was added to inactivate the proteases, the cell suspension was centrifuged at 500 rcf for 5 min.

    Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
    Article Snippet: Either HBSS with Calcium (14025092, Thermo Fisher Scientific Inc., Waltham, MA, USA) or without Calcium (14175095, Thermo Fisher Scientific Inc., Waltham, MA, USA) were used in the respective experiments, further supplemented with 2mM CaCl 2 or with 2 mM EGTA.

    Cell Culture:

    Article Title: Fasciola hepatica products can alter the response of bovine immune cells to Mycobacterium avium subsp. paratuberculosis
    Article Snippet: Following euthanasia by intra‐jugular administration of pentobarbital sodium (Euthanal 200 mg/mL, Merial Animal Health), lymph nodes were extracted during post‐mortem examination and suspended in ice‐cold Hank’s balanced salt solution (HBSS; without calcium or magnesium) supplemented with 1% antibiotic–antimycotic solution (Thermo Fisher Scientific).

    Article Title: STING signaling sensing of DRP1-dependent mtDNA release in kupffer cells contributes to lipopolysaccharide-induced liver injury in mice
    Article Snippet: A 26G catheter was inserted into the mouse portal vein, and the liver was perfused with 30 mL warmed Hank’s balanced salt solution (HBSS, without calcium or magnesium, GIBCO) containing 1% penicillin streptomycin to flush out the blood, followed by 40 mL DMEM/F12 (GBICO) containing 0.05% collagenase IV (GBICO) and 1% penicillin streptomycin.

    Article Title: NeuriteNet: A Convolutional Neural Network for determining morphological differences in neurite growth
    Article Snippet: Lumbar DRGs were harvested into NeurobasalTM media (Cat #21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), Cat #17100, ThermoFisher) for 40 min. After addition of 10% FBS to inactivate the proteases, the cell suspension was centrifuged at 201 rcf for 5 min.

    Article Title: Caldendrin represses neurite regeneration and growth in dorsal root ganglion neurons
    Article Snippet: DRGs (20–30) were harvested in NeurobasalTM media (Cat#21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin–EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), (Cat #17100, ThermoFisher) for 40 min. After the enzyme incubation, 10% horse serum was added to inactivate the proteases, the cell suspension was centrifuged at 500 rcf for 5 min.

    Article Title: NeuriteNet: A convolutional neural network for assessing morphological parameters of neurite growth
    Article Snippet: Lumbar DRGs were harvested into NeurobasalTM media (Cat #21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (−/−)), Cat #17100, ThermoFisher) for 40 min. After addition of 10% FBS to inactivate the proteases, the cell suspension was centrifuged at 168 × g for 5 min.

    Article Title: Caldendrin represses neurite regeneration via a sex-dependent mechanism in sensory neurons
    Article Snippet: 20-30 DRGs were harvested into NeurobasalTM media (Cat#21103049, ThermoFisher) and DRGNs were dissociated in 0.125% Trypsin-EDTA (Cat #25200056, ThermoFisher) and 0.1% collagenase type I (1 mg/mL in HBSS without calcium or magnesium (-/-)), (Cat #17100, ThermoFisher) for 40 min. After the enzyme incubation, 10% horse serum was added to inactivate the proteases, the cell suspension was centrifuged at 500 rcf for 5 min.

    Article Title: Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment
    Article Snippet: Either HBSS with Calcium (14025092, Thermo Fisher Scientific Inc., Waltham, MA, USA) or without Calcium (14175095, Thermo Fisher Scientific Inc., Waltham, MA, USA) were used in the respective experiments, further supplemented with 2mM CaCl 2 or with 2 mM EGTA.



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